Serodiagnosis of contagious bovine pleuropneumonia by immunoblotting
نویسنده
چکیده
Reagent production: selecting the strain, preparing the antigen and controlling quality The choice of the strain to use is an extremely critical point. The use of the B103 strain, the reference strain used by Regalla et al. in 1999 to develop the IBT method, is highly recommended and should be used to ensure consistent results among laboratories. The antigen is then prepared from a culture grown in mycoplasma broth medium (Poumarat et al., 1991) and concentrated to 1011 to 1012 CFU/mL (Table 1). It is absolutely essential to ensure that the cultured strain correctly expresses the five specific proteins targeted in the IBT, which are 110, 98, 95, 60-62 and 48 kDa in size, as defined by Gonçalves et al. (1998). The expression levels of these proteins can vary according to the MmmSC strain used and the selected clone. Thus, prior to use, each antigen batch must be assessed with positive and negative reference sera (Table 1). The culture must be checked for contamination by other mycoplasmas. Trace levels of contamination can be detected using the colony blot method (Gaurivaud et al., 2004) with a MmmSC-specific monoclonal antibody (Brocchi et al., 1993). The mycoplasma protein concentrate can be aliquoted and stored at -20°C up to one year.
منابع مشابه
Manifestation and epidemiology of contagious bovine pleuropneumonia in Europe.
The authors describe the clinical profile and epidemiology of contagious bovine pleuropneumonia in Europe. This disease, once considered to have been eradicated several years ago, has now become endemic in southern countries of Europe. The status of contagious bovine pleuropneumonia in Portugal and Italy, and the evolution of the disease during the last ten years, are analysed in detail, in add...
متن کاملContagious Bovine Pleuropneumonia
Contagious bovine pleuropneumonia (CBPP) is one of the most important infectious diseases of cattle in Africa. Naïve herds can experience losses up to 80%, and many cattle that survive remain chronic carriers. These carriers may suffer from recurrent low-grade fever, loss of condition, and respiratory signs upon exercise, and might introduce the virus into uninfected herds. Although contagious ...
متن کاملContagious Bovine Pleuropneumonia
Contagious bovine pleuropneumonia (CBPP) is one of the most important infectious diseases of cattle in Africa. Naïve herds can experience losses up to 80%, and many cattle that survive remain chronic carriers. These carriers may suffer from recurrent low-grade fever, loss of condition, and respiratory signs upon exercise, and might introduce the causative agent into uninfected herds. Although c...
متن کاملWhole-Genome Sequencing of Mycoplasma mycoides subsp. mycoides Italian Strain 57/13, the Causative Agent of Contagious Bovine Pleuropneumonia
Mycoplasma mycoides subsp. mycoides is generally considered one of most pathogenic Mycoplasma species, and it is the etiological agent of contagious bovine pleuropneumonia (CBPP). Here, we present the annotated genome sequence of M. mycoides subsp. mycoides Italian strain 57/13, isolated in 1992 during CBPP outbreaks in Italy.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2012